What is the difference between PCR and DNA replication?
If you're seeing this message, it means we're having trouble loading external resources on our website. Show
If you're behind a web filter, please make sure that the domains *.kastatic.org and *.kasandbox.org are unblocked. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. AbstractIn principle, PCR generates large quantities of DNA from a minute amount of nucleic acid starting material using a methodology similar to (but much simpler than) that seen in living cells. For living cells, in vivo DNA synthesis is dependent upon a well defined but complex set of enzymes and co-factors, which have evolved to act in a concerted fashion during the synthetic phase (S-phase) of the cell cycle. In comparison, PCR facilitates in vitro DNA synthesis in a much simpler fashion, making use of a smaller set of defined ingredients and reaction conditions involving relatively high temperatures. The range of factors contributing to successful PCR amplification is reviewed below. Keywords: Exonuclease Activity, Deoxynucleotide Triphosphate, Exonuclease Domain, Concerted Fashion, Single Strand Binding Protein References
|